Establishment of TUSMi005-A, an induced pluripotent stem cell (iPSC) line from a 32-year old Chinese Han patient with Bipolar Disorder (BD)
Establishment of TUSMi005-A, an induced pluripotent stem cell (iPSC) line from a 32-year old Chinese Han patient with Bipolar Disorder (BD)
Blog Article
A 32-year old Bipolar Disorder (BD) male patient Isabella Bird’s Unbeaten Tracks in Japan ou le récit de voyage comme autoportrait d’une aventurière engagée donated his Peripheral blood mononuclear cells (PBMC).The non-integrating episomal vector system used to reprogram PBMCs with the human OKSM transcription factors.The pluripotency of transgene-free iPSCs was confirmed by immunocytochemistry for pluripotency markers and by the ability of the iPSCs to differentiate spontaneously into 3 germ layers in vitro.In addition, the iPSC line displayed a normal karyotype.Our model might offer a good platform to further study the pathological mechanisms, to identify early biomarkers, and also for drug testing studies in BD.
Resource tableUnlabelled TableUnique stem cell line identifierTUSMi005-AAlternative name(s) of stem cell lineN/AInstitutionTongji University School of Medicine, Shanghai, Peoples R ChinaContact information of distributorYing Wang, [email protected] of cell lineiPSCOriginHumanAdditional origin infoAge: 32Sex: Ecology of parasites of Metynnis lippincottianus (Characiformes: Serrasalmidae) from the eastern Amazon region, Macapá, State of Amapá, Brazil maleEthnicity: Chinese HanCell sourcePeripheral blood mononuclear cells (PBMCs)ClonalityClonalMethod of reprogrammingTransgene free, Episomal plasmidsGenetic modificationNOType of modificationN/AAssociated diseaseBipolar Disorder (BD)Gene/locusSLC1A3/chr 5p13.2: 36679820, rs117588697 A-G,MAPT/chr 17q21.31:44073818, rs577501443 G-A,SIRT1/chr 10q21.3: 67864422, rs12415800 G-AMethod of modificationN/AName of transgene or resistanceN/AInducible/constitutive systemN/ADate archived/stock date08th July,2018Cell line repository/bankhPSCreg, https://hpscreg.
eu/user/cellline/edit/TUSMi005-AEthical approvalParticipants informed consent obtained/Ethics Review Board-competent authority approval obtained (EC.D(BG).016.01.1).